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C3a effects on COX-2 in 3T3-L1, B16/F0 and Raw 264.7 Blue cells were stimulated with CHO F3 clone supernatants (2% and 20%) during a 24 h period. Ct cells are cells treated with conditioned medium from non-transfected CHO. COX-2 expression was determined by qRT-PCR. Reported values are means ± SEM of three independent experiments. (* p < 0.05, *** p < 0.001, **** p < 0.0001 as compared to untreated cells (Ct)).
