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. 2023 Jun 4;12(11):1542. doi: 10.3390/cells12111542

Figure 2.

Figure 2

The functional characterization of Arabidopsis transgenic lines carrying EaF82 promoter driving EaF82-sGFP (TA) or GUS. (a) The newly opened flower (left) shows GFP signal in anthers (right). A: anther. (b) Confocal microscopy shows GFP (left) and DIC (differential interference contrast, right) in stamen and some pollen grains. S: stamen; PG: pollen grains; Bar = 100 µm. (c) GUS staining of the flowers of EaF82p::GUS. White arrow indicates GUS activity at the early developmental stages of flowers. Bar = 1.5 mm. (d) Immunoblot of pollen proteins against anti-EaF82 and anti-GFP antibodies. Stained blots show protein loading. TA-1, -3, -4, and -5: four independent lines. TB-2: Proteins isolated from flowers of Arabidopsis transgenic 35Sp::EaF82-sGFP. B: blank. C: vector control. WT: wild type. M: protein size marker. (e) Confocal microscopy detecting GFP in collected pollen grains from Arabidopsis transgenic EaF82p::EaF82-sGFP (TA) plants. WT is a negative control. Bar = 100 µm. (f) A 10-week-old TA plant (right) bears aborted siliques in primary inflorescence stalk (yellow bracket) compared to normal WT (left). Bar = 1 cm. (g) Collected pollen grains from fully opened flowers stained with iodine-potassium iodide. (h) Germinated pollen under germination medium. Bar = 100 µm.