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. 2023 May 25;24(11):9234. doi: 10.3390/ijms24119234

Figure 4.

Figure 4

Effect of Ceramide AD™ on HDM-induced MMP-9 activity. (A) Expression of the active form of MMP-9 in primary human keratinocytes (KHN) stimulated with HDM 100 μg/mL for 24 h in the presence or absence of Ceramide AD™ at 1/20, 1/50 or 1/100 dilution dissolved in 5% glycol. WB is a representative gel of 3 separate experiments. (B) MMP-9 activity in the supernatant was measured by ELISA following HDM stimulation of human skin biopsies with (green) or without (grey) cream containing Ceramide AD™ at 0.08% or 0.1% or using MMP-9 inhibitor Ab142180. Placebo (or vehicle control) was the same cream but did not contain Ceramide AD™ component (n = 3 separate experiments). Differences between groups were tested using Wilcoxon Signed Rank Test. ** p < 0.01 and *** p < 0.001.