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. 2023 May 24;24(11):9181. doi: 10.3390/ijms24119181

Figure 3.

Figure 3

rMASP-1 cooperated with LPS in the induction of E-selectin on endothelial cells. Confluent layers of HUVECs were cultured in 96-well plates. (A): The cells were pretreated with LPS (10 ng/mL) for 24 h, and after the removal of the pretreatment, rMASP-1 (0.6 μM) was added to the cells for 6 h. (B): In the case of cotreatment, LPS (10 ng/mL) and rMASP-1 (0.6 μM) were added together to the cells for 6 h. Then, the cells were fixed, and the expression level of E-selectin was determined by cell-based ELISA. Compared to the control: control vs. rMASP-1: ns., control vs. LPS-pretreated control: ns., control vs. rMASP-1 treatment after LPS pretreatment: ****, control vs. LPS: ****, control vs. LPS + rMASP-1: ****; +: indicates that the pretreatment or treatment was added; -: indicates that pretreatment or treatment was not added; ns: non-significant; ** p < 0.01; *** p < 0.001; **** p < 0.0001.