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. 2023 May 24;24(11):9181. doi: 10.3390/ijms24119181

Figure 5.

Figure 5

rMASP-1 and LPS cotreatment increased endothelial permeability. Confluent layers of HUVECs were cultured in 96-well plates precoated with biotinylated gelatin. The cells were treated with LPS (10 ng/mL), rMASP-1 (0.6 μM), both, or with medium alone (control) for 24 h. Cell-free areas of the surface of biotinylated gelatin were stained with Streptavidin-Alexa488, and after fixation, fluorescence was detected with a fluorescence plate-reader; representative images were taken using an Olympus IX-81 fluorescence microscope. (A) Representative images of three independent experiments. The scale bar applies to all photos. (B) Mean values of three independent experiments normalized to the controls. Compared to the control: control vs. rMASP-1: ***; control vs. LPS: ****, control vs. rMASP-1 + LPS: ****; *** p < 0.001; **** p < 0.0001.