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. 2023 May 30;12(11):1516. doi: 10.3390/cells12111516

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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(A) Representative blots showing the expression of Mecp2, PSD-95 and Synaptophysin in NSCs following miR-26b-5p overexpression. β actin was used as an internal control. (B) Densitometry analysis shows significant downregulation in Mecp2 and PSD-95 proteins, and upregulation in Synaptophysin protein in NSCs following miR-26b-5p overexpression (closed bars) compared to that of negative control (open bars). Nc—Negative control, miR-26b OE—Overexpression of miR-26b-5p. n = 3, * p < 0.05. miR-26b-5p was overexpressed in NSCs from embryos of control pregnancy, and immunoexpression of Synaptophysin and PSD-95 was observed using confocal microscopy. (C,D) Confocal images show an increase in expression of Synaptophysin (C) and a decrease in expression of PSD-95 (D) in NSCs following miR-26b-5p overexpression (right panel) when compared to that of negative control (left panel). Scale bar, 10 μm. Nuclei are stained with DAPI. (E) Representative blots showing expression of Mecp2, PSD-95 and Synaptophysin in NSCs following Mecp2 knockdown. β actin was used as an internal control. (F) Densitometry analysis shows significant downregulation of Mecp2 and PSD-95 protein expression and upregulation of Synaptophysin protein expression in NSCs following Mecp2 knockdown. Nc—Negative control, Mecp2 KD—Knockdown of Mecp2. n = 4, ** p < 0.01, * p < 0.05. (G) Confocal images show an increase in Synaptophysin in NSCs following Mecp2 knockdown (right panels) when compared to that of negative control (left panels). Nuclei are stained with DAPI. Scale bar, 10 μm.

(A) Representative blots showing the expression of Mecp2, PSD-95 and Synaptophysin in NSCs following miR-26b-5p overexpression. β actin was used as an internal control. (B) Densitometry analysis shows significant downregulation in Mecp2 and PSD-95 proteins, and upregulation in Synaptophysin protein in NSCs following miR-26b-5p overexpression (closed bars) compared to that of negative control (open bars). Nc—Negative control, miR-26b OE—Overexpression of miR-26b-5p. n = 3, * p < 0.05. miR-26b-5p was overexpressed in NSCs from embryos of control pregnancy, and immunoexpression of Synaptophysin and PSD-95 was observed using confocal microscopy. (C,D) Confocal images show an increase in expression of Synaptophysin (C) and a decrease in expression of PSD-95 (D) in NSCs following miR-26b-5p overexpression (right panel) when compared to that of negative control (left panel). Scale bar, 10 μm. Nuclei are stained with DAPI. (E) Representative blots showing expression of Mecp2, PSD-95 and Synaptophysin in NSCs following Mecp2 knockdown. β actin was used as an internal control. (F) Densitometry analysis shows significant downregulation of Mecp2 and PSD-95 protein expression and upregulation of Synaptophysin protein expression in NSCs following Mecp2 knockdown. Nc—Negative control, Mecp2 KD—Knockdown of Mecp2. n = 4, ** p < 0.01, * p < 0.05. (G) Confocal images show an increase in Synaptophysin in NSCs following Mecp2 knockdown (right panels) when compared to that of negative control (left panels). Nuclei are stained with DAPI. Scale bar, 10 μm.