Table 6.

TA97a and TA98 revertant colonies detected after exposure to the three oils (coconut, rapeseed and grape seed) both without (0%) S9) and with metabolic activation (10% S9). Mean ± SD of three replicates is presented. Each of the oils were tested without any treatment (T0), after 10 and 20 days of treatment at 65 °C (T1 and T2, respectively) and after 90 min at 180 °C (T3). Positive controls (C+) were: (a) without metabolic activation (0%S9), 4-nitro-o-phenylenediamine (NPD) 10 µg/well for TA97a and 2 µg/well for TA98; (b) with metabolic activation (10% S9), 2-aminoanthracene (AA) 2 µg/well for TA97a, and 2-aminofluorene (AF) 10 µg/well for strain TA98.

	TA97a		TA98
	0%S9	10%S9	0%S9	10%S9
C−	36 ± 12	54 ± 5	8 ± 1	12 ± 1
C+	315 ± 32	421 ± 94	317 ± 43	877 ± 82
Coconut oil
T0	36 ± 5	73 ± 15	10.2	13 ± 5
T1	41 ± 11	57 ± 6	7 ± 1	8 ± 1
T2	33 ± 2	56 ± 3	10 ± 2	11 ± 2
T3	45 ± 3	73 ± 4	10 ± 2	10 ± 3
Rapeseed oil
T0	35 ± 3	73 ± 2	12 ± 2	13 ± 4
T1	61 ± 9	76 ± 9	10 ± 4	8 ± 3
T2	42 ± 1	70 ± 6	7 ± 5	15 ± 7
T3	52 ± 10	73 ± 3	10 ± 1	11 ± 2
Grape seed oil
T0	45 ± 4	73 ± 5	15 ± 3	12 ± 1
T1	47 ± 6	75 ± 11	13 ± 2	11 ± 2
T2	48 ± 7	72 ± 11	11 ± 3	10 ± 3
T3	39 ± 7	67 ± 9	11 ± 4	16 ± 11