Figure 4.
SSEA-4-directed CAR T cells induce varying levels of toxicity in tumor-free NSG mice. (A) Body weight development in naïve NSG mice following adoptive transfer of 1 × 107 XS, S, or L spacer CAR T cells. For the injection of untransduced control T cells, the cell number was adjusted to the highest total T cell number injected in the CAR T cell groups. (B) Blood serum levels of human IFNγ, IL-2, and TNFα in non-tumor-bearing NSG mice 5 days after systemic CAR T cell treatment (n = 3/group). A non-parametric Mann–Whitney U test was used for statistical comparison, and no statistical significance was observed. *, p < 0.05; **, p < 0.01; ***, p < 0.001.