Figure 3.

Splicing timecourses comparing ultrapure KCl (Aldrich and AlfaAesar) to standard KCl (Fisher), KAc and KGlu. Full-length AdML substrate was incubated under standard splicing conditions (80 mM potassium) using partial substitution to add the various potassium counterions. Splicing time points were 0, 15, 30, 60 and 90 min. RNA species are indicated to the left of the gel. Observed amounts of ligated exon product versus time are shown to the right. Values for ligated exon product represent raw counts taken from the PhosphorImager volume report. The asterisk (*) indicates that this point was discounted because lane 4 was clearly overloaded.