Figure 5.

Partial substitution of chloride with acetate or glutamate yields levels of spliced product comparable to microdialysis. Using a full-length AdML substrate, splicing was compared under the following conditions: (I) chloride nuclear extract with additional KCl and MgCl₂ in the splicing buffer; (II) chloride nuclear extract with KAc and MgAc₂ in the splicing buffer; and (III) acetate nuclear extract with KAc and MgAc₂ in the splicing buffer. The percentage of first step products shown to the right was calculated as described in Materials and Methods.