Figure 1.

PET imaging: Blocking of [¹⁸F]CPFPX uptake with rolofylline in brains of WT mice. Animals were injected intravenously with 10 mg/kg rolofylline together with 10 MBq [¹⁸F]CPFPX, a tracer that binds specifically to adenosine A1 receptors. (A,C) Average images of [¹⁸F]CPFPX SUVR_bg in horizontal (H) and sagittal (S) view, projected onto an MRI template in the absence of rolofylline (A) or injected with [¹⁸F]CPFPX + 10 mg/kg rolofylline (C). (B) Significant differences on the voxel level between the two measurements, analyzed using paired t-test corrected for multiple testing (p < 0.05). (D) Box plots of VOI values. * p < 0.05 when comparing to unblocked [¹⁸F]CPFPX uptake. (E) Analysis of the [¹⁸F]CPFPX SUVR_bg in different brain areas. Results are expressed as mean ± SD (n = 6/group). Abbreviations: Cer: cerebellum; MB: midbrain; WB: whole brain. Scale bar: 5 mm.