Figure 1.

In vitro migration ability of breast and lung cancer cells lines. In panel (A) are summarized data regarding the proportion of cells counted in the lower well of the Transwell chamber expressed as % of cell transmigrated (see M&M). Although not significant, a trend of inverse correlation between transmigration ability and the rate of expression of adhesion molecules ICAM1 and a2 integrin in particular was detected. Cells attached to the lower side of the Transwell, i.e., cells which have been able to the cross endothelial cell monolayer-coated polycarbonate membrane, but that have remained attached to the lower face of the Transwell, are reported in panel (B). Higher adhesion molecule expression of the specific cell line directly correlated with the number of cells trapped (Figure S2). Data are expressed as n. of cells/field (40×). Correlations between migration parameters and ICAM1 or α2-integrin expression indexes are reported on the right side of panels (A,B). Data refer to at least two independent experiments. Error bars represent the standard deviations.