Figure 6.

Impact of TFN on AZA-sensitive and AZA-resistant cell variants after 72 h of cultivation. TFN was added to the culture medium every 24 h at four concentrations (3, 4, 5, and 6 μM). Bars for DMSO represent the cells treated with the highest concentration of DMSO present during TFN treatment. (A) Relative number of viable cells after TFN treatment compared to untreated controls as measured by CASY TT. (B) Relative metabolic activity of cells after TFN treatment as measured by MTS assay. (C) Proportions of viable (unstained), apoptotic (stained with Annexin-V or both Annexin-V and PI), and necrotic (stained by PI) cells.