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. 2023 May 23;24(11):9149. doi: 10.3390/ijms24119149

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Immunohistochemical analysis of tumor heterotransplants derived from UROtsa As_I cells. (A) Nuclear SOX2 expression in the tumors produced by the As_I cells, SOX2 was expressed both in the well-differentiated areas (squamous, *) and the non-differentiated areas (basal, ^). (B) Cytoplasmic IVL expression, IVL appears to be mainly expressed in the well-differentiated/squamous areas (*) and mostly absent in basal areas (^). (C) Nuclear GRHL1 expression, GRHL1 appears to be mainly expressed in the well-differentiated/squamous areas (*) and mostly absent in basal areas (^). The brown color indicates the presence of the protein whereas the blue/purple color indicates the nuclei that are stained with the counterstain hematoxylin. All images are at a magnification of 200×.