Table 1.
Measured concentrations (mean value ± SD) of pteryxin and VPA/VPN in larvae whole-body (pooled n = 18) after 1 h and 18 h immersion quantified in three independent experiments using LC-MS/MS. Larvae were washed prior to homogenization.
| Treatment/Concentration/Time | [ng/g] | [µM] | % Absorbed |
|---|---|---|---|
| Pteryxin/1 µM/1 h | 468.7 ± 44.1 | 1.2 ± 0.1 | 1.2% |
| Pteryxin/10 µM/1 h | 1243.3 ± 201.3 | 3.2 ± 0.5 | 32.2% |
| Pteryxin/20 µM/5 min | 820.0 ± 46.3 | 2.1 ± 0.1 | 10.1% |
| Pteryxin/20 µM/30 min | 2530.0 ± 105.4 | 6.5 ± 0.3 | 32.7% |
| Pteryxin/20 µM/1 h | 4280.0 ± 284.8 | 11.1 ± 0.7 | 55.4% |
| Pteryxin/20 μM/18 h | 3406.7 ± 213.6 | 8.8 ± 0.5 | 44.0% |
| Pteryxin/20 μM/without larvae | 4090.6 ± 381.6 | 20.5 ± 0.1 | - |
| VPN/VPA 100 µM 1 h | 940.7 ± 40.8 | 6.5 ± 0.3 | 6.5% |
| VPN/VPA 500 µM 1 h | 1680.0 ± 30.0 | 11.6 ± 0.2 | 2.3% |
| VPN/VPA 1 mM 1 h | 4740.0 ± 111.4 | 32.9 ± 0.8 | 3.3% |
| VPN/VPA 5 mM 5 min | 5596.6 ± 289.9 | 38.8 ± 2.0 | 0.8% |
| VPN/VPA 5 mM 30 min | 10,366.7 ± 472.6 | 71.9 ± 3.3 | 1.4% |
| VPN/VPA 5 mM 1 h | 18,166.7 ± 404.1 | 126.0 ± 2.8 | 2.5% |
| VPN/VPA 10 mM 1 h | 33,933.3 ± 1457.2 | 235.3 ± 10.1 | 9.4% |
| VPN/VPA (5 mM) 18 h | 5473.33 ± 225.02 | 37.97 ± 1.57 | 0.76% (metabolism) |
| VPN/VPA 5 mM without larvae | 734,333 ± 5859 | 5092 ± 40.6 | 100% |
| Medium control | No detected | Not detected | - |