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. 2023 May 25;24(11):9280. doi: 10.3390/ijms24119280
Actb gene encoding β-actin
ANOVA analysis of variance
cDNA complementary DNA
DAB 3,3′-diaminobenzidine tetrahydrochloride
DAPI 4,6-diamidino-2-phenylindole dihydrochloride
DTT dithiothreitol
E2 17β-estradiol
EDTA ethylenediaminetetraacetic acid
ERE estrogen response element
ERα estrogen receptor-α
ERβ estrogen receptor-β
Esr1 −/− estrogen receptor 1 knockout
FSH follicle-stimulating hormone
Fshb −/− follicle-stimulating hormone β knockout
GAPDH glyceraldhyde-3-phosphate dehydrogenase
GPER1 G protein-coupled estrogen receptor 1
H & E hematoxylin and eosin
Il1b interleukin 1β
Itgam integrin αM
LCN2 lipocalin 2
LDS lithiumdodecyl sulfate
LH luteinizing hormone
MES 2-(N-morpholino) ethanesulfonic acid
Mpo myeloperoxidase
NGAL neutrophil gelatinase-associated lipocalin
PBS phosphate buffered saline
PCOS polycystic ovary syndrome
Rps6 ribosomal protein S6
RT room temperature
RT-qPCR reverse transcription and quantitative real-time PCR
STAR steroidogenic acute regulatory protein
TBS-T Tris-buffered saline with Tween 20
Tnfa tumour necrosis factor-α
WT(s) wild-type(s)