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Cell viability assay with HT115 DE3 cells resuspended in reduced volume of 0.1% (w/v) SDS and boiled at 95 °C for 2 min (a) compared to cells resuspended in 2% (w/v) LB-medium without boiling and incubated at room temperature (b,c). Plated were 1 µL of undiluted cell suspension in (a,b), and 10 µL of 1:100 dilution in (c).
