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. 2023 May 27;24(11):9354. doi: 10.3390/ijms24119354

Figure 7.

Figure 7

PebHLH35 bind to the PeGSTU58 promoter and activates its expression. (A) The location of amplified promoter region (Pro) of PeGSTU58 in its promoter. (B) A diagram of vectors used for yeast one-hybrid analysis. (C) Yeast one-hybrid analysis of interactions between PebHLH35 and PeGSTU58 promoter. The different combination of reporter and effector constructs were co-transformed into yeast Y187 cells, the positive control (p53HIS2 + pGAD-53), and the negative control (p53HIS2 + pGADT7-PebHLH35); PeGSTU58∆pro means the promoter of PeGSTU58 without “TCAACTTGA” motif. (D,E) A diagram of vectors used for transient dual-luciferase assays (D). The different combination of effector and reporter vectors were instantaneously co-transformed into N. benthamiana leaves. An image of N. benthamiana leaf with fluorescence was captured (E).