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. 2023 May 26;24(11):9322. doi: 10.3390/ijms24119322

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Cloning and transformation of StNRAMP2: (A) shows the PCR results of Agrobacterium Tumefaciens colonies (M: DL2000; 1–6: pTRV2 + StNRAMP2 gene; 7: plasmid; 8: water); (B) shows the expression of TRV-StNRAMP2 gene silenced plants. In the figure, * means significant difference p < 0.05, and ** means extremely significant p < 0.01. (C) shows the colony PCR detection of the part-CAM-FLAG-StNRAMP2 plasmid, M is the DL2000 marker, and 1–4 are the colony PCR results of the target gene. (D) Genetic transformation process of part-CAM-FLAG-StNRAMP2 tomato, 1: Preparation of explants; 2: callus formation; 3: resistant bud formation; 4: rooting culture.