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(a) Analysis of ARSA enzymatic activity in HEK293T cell lysates following AAV9-ARSA transduction. (b) Western blot analysis of ARSA proteins in native and genetically modified HEK293T AAV9-ARSA cells. (c) Determination of overall purity of AAV-ARSA using SDS-PAGE protein electrophoresis, where VP1, VP2, and VP3 are viral proteins. Data are presented as means ± S.D. ****—p < 0.0001.
