Table 2.

Summary of some characteristics of the most common DNAPs employed in WGA methods. Translesion DNA synthesis (TLS) activity is indicated as the blocking damaged bases that DNAP can synthesize opposite to, corresponding to tetrahydrofuran (THF) (an abasic site analog), thymine glycol (Tg), and thymine dymer (T:T). ND: no data available.

DNA Polymerase	Family	Processivity	5′-3′ Exo	3′-5′ Exo	Strand Displacement	Average Error Rate	TLS	Optimum Temp. (°C)
Taq	A	20–50 nt [78,79]	+/− c	−	−	1–2 × 10−4	THF [80]	70–75
Pwo	B	<Taq [81] a	−	+	−	2.4 × 10−6	ND	72 b
Bst	A	<Φ29DNAP [73] a	+/− c	−	+	1.5 × 10−5	ND	65
Φ29DNAP	B	>40 Kb [33,35,73]	−	+	+	5 × 10−6	[74]	30
B35DNAP	B	>23 Kb [74]	−	+	+	5 × 10−6	THF, Tg, T:T [74,82]	37

^a Only approximate comparative references for processivity are available. ^b Data has been extracted from the temperature use in diverse scientific publications and commercial protocols [83,84]. ^c 5′-3′ exonuclease activity present in wild-type but absent in the commonly used Klenow large fragment.