Skip to main content
. 2023 Jun 4;12(11):1543. doi: 10.3390/cells12111543

Figure 5.

Figure 5

Live cell imaging analysis of PDE2 and PDE3 inhibitor effects in adult cardiomyocytes. The adult rat ventricular cardiomyocytes were transduced for 6 days with Cas9 or Cas9 plus gRNA adenoviruses as described in Figure 4. The effect of the PDE2 inhibitor BAY 60-7550 (BAY, 100 nM) in the PDE2A-KO cells (A–C) and the effect of the PDE3 inhibitor Cilostamide (Cilo, 10 µM) in the PDE3A-KO cells (D–F) were measured after beta-adrenergic stimulation with 100 nM ISO as described in Figure 3. Representative FRET traces and data analysis for ISO, BAY, and Cilo responses are shown. The p-values were from a mixed ANOVA followed by a Chi-squared test; n.s.—not significant.