Figure 1.

Protein–protein interactions monitored with the use of a universal protein array. (A) Autoradiographic signals detected from the array that was incubated with the ³²P-labeled GST-K–p52 and washed with buffer A100. (B) Signals detected after washing the array (from A) with buffer A1000. (C) Quantitative representation of relative affinities (in reading units from a densitometer) of 48 proteins for the transcriptional coactivator p52 after washing with 1000 mM KCl.