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. 2000 Jan 15;28(2):e3. doi: 10.1093/nar/28.2.e3

Figure 3.

Figure 3

Use of UPA to detect ASF/SF2-interacting proteins. (A) Sixteen selected proteins (or fractions) were analyzed for interaction with 32P-labeled 6H(K)ASF/SF2. CTD, the C-terminal domain of RNA polymerase II fused to GST; RPB5, RPB6, RPB8, RPB10α and RPB10β correspond to individual subunits of RNA polymerase II fused to GST; TBP, TATA-binding protein; f:TFIID, affinity-purified flag-tagged TBP-containing TFIID complex from HeLa cells; RXR, retinoid-X receptor; TR, thyroid hormone receptor; His-H1, histone H1; Co-His, co-histones; HMG1, high mobility group protein 1; ASF, alternative splicing factor; GST–Nu, GST–nucleolin fusion; GST-K, GST fused with a synthetic heart muscle kinase site. (B) Autoradiographic detection of UPA after washing the membrane with 100 mM KCl. (C) Signals detected after washing the membrane with 500 mM KCl.