Figure 5.

Detection of flavin mononucleotide and NADH release in the perfusate by fluorescence. FMN release was comparable between all study group at 240 and 270 min of HMP. At the end of machine perfusion FMN, values were significantly higher in the continuously membrane-oxygenated group as compared to both surface-oxygenated study groups (p = 0.0012) (a). At each timepoint, NADH release in the circulating perfusate detected by fluorescence was significantly higher in the intermittently oxygenated HMP group as compared with the continuously surface-oxygenated HMP group but comparable to the membrane-oxygenated group (b). FMN, flavin mononucleotide; HMP, hypothermic machine perfusion; NADH, reduced nicotinamide adenine dinucleotide.