Figure 6.
Metabolic tissue analysis by liquid chromatography electrospray ionization mass spectrometry demonstrated that lactate levels measured at 4 h 30 of HMP were significantly lower in the intermittent surface-oxygenated group as compared with both continuously oxygenated groups but were comparable between all groups at the end of the preservation period (a). No differences in glutamate, succinate, ATP, NADH, NAD+, and FMN level were observed in biopsies obtained at 4 h 30 and at the end of perfusion, comparing all study groups (b–g). ATP, adenosine triphosphate; FMN, flavin mononucleotide; HMP, hypothermic machine perfusion, NADH, reduced nicotinamide adenine dinucleotide; NAD+, oxidized nicotinamide adenine dinucleotide.