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. 2023 May 29;12(11):3731. doi: 10.3390/jcm12113731

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Metabolic tissue analysis by proton NMR demonstrated comparable concentrations of lactate, glutamate, ATP, and aspartate, during and at the end of machine perfusion. At the end of HMP, succinate was significantly higher in the intermittent surface-oxygenated group as compared with the continuous membrane-oxygenated group but comparable with the continuously surface-oxygenated group. (a–e) Lactate as a marker of anaerobic metabolism as well as the regeneration of ATP, and glutamate and aspartate were comparable between all the study groups at the end of machine perfusion. ATP, adenosine triphosphate; HMP, hypothermic machine perfusion; ns, not significant.

Metabolic tissue analysis by proton NMR demonstrated comparable concentrations of lactate, glutamate, ATP, and aspartate, during and at the end of machine perfusion. At the end of HMP, succinate was significantly higher in the intermittent surface-oxygenated group as compared with the continuous membrane-oxygenated group but comparable with the continuously surface-oxygenated group. (a–e) Lactate as a marker of anaerobic metabolism as well as the regeneration of ATP, and glutamate and aspartate were comparable between all the study groups at the end of machine perfusion. ATP, adenosine triphosphate; HMP, hypothermic machine perfusion; ns, not significant.