Skip to main content
. 2023 Jun 2;24(11):9696. doi: 10.3390/ijms24119696

Figure 6.

Figure 6

SPINK2 knockdown modulates expression of immune-response related genes in LSC-like cells. (A) ALCAM mRNA expression by qPCR in KG1a, ME1 and GDM1 cells with negative control siRNA (#neg) and SPINK2 knockdown with 2 siRNAs (#1 and #2). Statistics: one-way ANOVA with Dunnett’s multiple comparison’s test. Mean ± SD is shown for two independent experiments. (B) Western blots showing ALCAM expression in KG1a, ME1 and GDM1 cells with negative control siRNA (#neg) and SPINK2 knockdown with 2 siRNAs. The numbers denote the relative protein expression normalized to the loading control. GAPDH was used as loading control. (C) mRNA expression by qPCR of several genes consistently downregulated by SPINK2 knockdown in KG1a cells. Blue arrows indicate T-cell activity inhibitory genes (NQO1, CD86, S100A9) which were downregulated ≥ two-fold. Statistics: one-way ANOVA with Dunnett’s multiple comparisons test. Mean ± SD is shown for three independent experiments. For all images: * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.