Table 2. Branch migration during a yeast DNA extraction protocol using SDS.

Treatmenta	Branch migration (%)b
	No addition	+ Co3+(NH3)6Cl–3c
Spheroplasting	85	0
SDS lysis	85	68
Phenol:chloroform	73	50
Chloroform	0	n.d.
Isopropanol	0	0
Ethanol	0	0

n.d., not determined.

^aAnnealed substrates were aliquoted into the following: spheroplasting buffer or SDS lysis buffer, and incubated at 37°C for 30 min; TNM buffer, and extracted with phenol:chloroform or chloroform; 0.3 M sodium acetate, and precipitated using isopropanol or ethanol.

^bBranch migration using 300 bp substrates S1 and S2 was measured as in Figure 2.

^cSolutions were supplemented with 10 mM Co³⁺(NH₃)₆Cl^–₃ as indicated. Phenol, but not chloroform, was equilibrated with buffer containing 10 mM Co³⁺(NH₃)₆Cl^–₃.