Table 2.
Phenolic acids and metabolites identified in the urine samples of healthy subjects by HPLC–ESI–MS/MS following the juçara juice intake.
| Identified Compounds (Negative Mode) |
RT (min) |
[M − H+]− (m/z) |
MS2 (m/z) |
|---|---|---|---|
| Protocatechuic acid a | 7.6 | 153 | 109 |
| Vanillic acid glucuronide | 8.4 | 343 | 167 |
| Hippuric acid a | 13.6 | 178 | 134 |
| Vanillic acid a | 14.2 | 167 | – |
| Hydroxybenzoic acid a | 12.0 | 137 | – |
| Hydroxyphenylacetic acid a | 12.8 | 151 | – |
| Kaempferol glucuronide | 14.3 | 463 | 287 |
| Ferulic acid derivate | 23.6 | 193/175 | 193/175 |
a Compound identity was confirmed with commercial standards. RT, retention time; MS2, two tandem mass spectrometry.In the present study, parent anthocyanins were not detected in urine, only their metabolites. The primary anthocyanin metabolites excreted were phenolic acids. Seven phenolic acids and one flavonol were identified in urine (Table 3).