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. 2023 May 30;24(11):9516. doi: 10.3390/ijms24119516

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Graphical representation of tissue processing and quantitative microanatomy. Panel (1) shows the route of venom administration: (A) intravenous, intradermal (B), and intramuscular (C). Panel (2) shows tissue collecting and processing: kidney and gastrocnemius muscle (D), followed by (E) microtome sectioning. Panel (3) shows the dyes used for staining the kidney ((F) haematoxylin and eosin) and gastrocnemius muscle ((G) Masson’s trichrome). Panel (4): (H) Quantification of tissue injury was performed via microscopic analyses. Created with BioRender.com.