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Chromatogram of standard rutin 10 ppm mobile phase containing H2O-acetonitrile in 0.1% of TFA. Separation column: monolithic column C18 (4.6 × 150 mm I.D.); injection loop: 20 μL; flow rate: 1.5 mL/min; temperature 30 °C; detection by absorbance at 340 nm; black arrow shows the peak of rutin (11.5 min).
