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. 2023 May 30;28(11):4433. doi: 10.3390/molecules28114433

Figure 3.

Figure 3

LOXL2’s SRCR-1 domain is required for protein interaction: (A) Schematic representation of wild-type LOXL2 (WT) and mutants carrying a deletion of the following SRCR domains: SRCR-1 (Δ-1), SRCR-2 (Δ-2), SRCR-3 (Δ-3), or SRCR-4 (Δ-4). (B) Whole-cell lysates of HEK293T cells transfected with LOXL2 WT or indicated SRCR-deletion mutants were immunoprecipitated with anti-ELAVL1/HuR antibody and analyzed by WB, with anti-LOXL2 antibody and anti-HuR antibodies as controls. (C) HEK293T cells were transfected with LOXL2 (WT) or the mutant carrying the deletion of the SRCR-1 domain (Δ-1); LOXL2 was immunoprecipitated using anti-HA antibody and analyzed by WB with the indicated antibodies. (D) The activity of the E-cadherin promoter in HEK293T cells was measured in the presence of the indicated LOXL2 mutants. The activity was determined as relative luciferase units (RLU) and normalized to the activity detected in the presence of the control pcDNA3 vector. Results represent the mean ± SEM. of at least three independent experiments performed in triplicate (*** p < 0.001, ns, not significant).