Figure 1.

Screening and characterization of phage-resistant mutants of strain 614F. (A) Screening procedures. (B) Ten-fold dilution of lysates of Yep-phi applied to bacterial lawns of 614F wild-type and phage-resistant strains. The maximum titer of bacteriophage is 10⁸ PFU. (C) The efficiency of plating (EOP) of Yep-phi phage on various strains. EOP = (number of PFUs on mutant strain)/(number of PFUs on wild-type strain). The titer of bacteriophage is 1.68 × 10⁹ PFU. (D) Adsorption of Yep-phi on strain 614F and its derivates. The Y-axis represents percentage of residual plaque-forming unit (PFU). Error bars show standard deviations of the mean of three biological replicates. Significance was determined by one-way ANOVA followed by Dunnett’s multiple comparison test. ^# P< 0.0001. (E) Adsorption kinetics of Yep-phi to strain 614F and its derivates. ***P< 0.001 (two-way RM ANOVA with Dunnett’s multiple comparison test). Error bars indicate standard deviations in triplicate samples.