Figure 8.

Expression of survival genes and cell cycle transcription factors in auranofin- or palbociclib-treated mesothelioma cell lines. Western blots showing representative expression patterns of pro-apoptotic (Bax and Bid) and pro-survival (Bcl-xL, Mcl-1, survivin, and XIAP) following 72 h of treatment from 2 or 3 independent experiments. No survivin signal was detected in the auranofin-treated H2452 cell line despite repeated attempts and the signal in the palbociclib-treated cells required extended duration ECL for detection. Actin was used as the lane loading control and is shown at bottom and represents the signal observed for the Western blots used for the figure. The cell lines and treatments are indicated above the blots. The proteins examined are indicated on the left. AU, auranofin; PD, palbociclib.