Figure 4.

CNT length and wettability do not induce indirect cytotoxicity in HaCaT cells. Extracts were prepared from different scaffolds by incubating scaffolds with Simulated Wound Fluid (SWF) for 14 days, and a 50% extract and complete media mixture were evaluated for potential cytotoxicity on Day 3 on normally dividing and quiescent HaCaT cells by the LDH assay. Scaffolds included CFC, Long CNT-CFC, and Short CNT-CFC with or without sodium hypochlorite-based bleach treatment. The Y-axis represents the quantification of mean formazan-correlated cytotoxicity results normalized to positive cytotoxic controls. The X-axis represents the treatment groups. Statistical significance was measured by two-way ANOVA analysis followed by Bonferroni post-tests. No significant differences compared to the cell control were observed. Results are representative of two independent experiments (n = 3 for each treatment group). Error bars denote SE values.