Figure 8.

CNT-coated scaffolds provide protection from Ultraviolet-B (UVB) exposure. Cells were labeled with CellTrace™ CFSE, cultured on scaffolds, and incubated for 1 day. Scaffolds were then imaged for cell counts by capturing 6 × 6 stitched images using Agilent BioTek Cytation5. Once imaged, scaffolds were treated with 100 J/m2 UVB and further incubated for 3 days. The scaffolds were again imaged by capturing 6 × 6 stitched images using Agilent BioTek Cytation5. Cell counts were calculated by using the Gen5 software. CNT-coated scaffolds retained a significantly increased number of cells after UVB treatment compared to the cell control, where cells were grown on a cover slide. The upper and lower rows are fluorescent images of cells grown on cover slides and represent cell control, carbon fiber cloth (CFC) control, Long CNT-coated scaffold, and Short CNT-coated scaffold before and after UVB treatment, respectively (A). The scale bar represents 3000 μm. Y-axis represents the relative cell number per each group after UVB treatment compared to the cell numbers of the same group before UVB treatment (B). Statistical significance was measured by one-way ANOVA with Tukey post hoc analysis. Significant difference from the cell control is denoted by *, **, and ***, which represents significance at p < 0.05, p < 0.01, and 0.001, respectively. Results are representative of three separate experiments (n = 3 for each treatment group). Error bars denote SE.