Figure 9.

Scaffolds may enhance antibody secretion in stimulated B cells. CL-01 cells (A,B) and SKW cells (C) were incubated for 4 days with or without hydrophilic long or short CNT scaffolds. Levels of IgG (A) and IgM (B,C) were quantified via enzyme-linked immunosorbent assay (ELISA). Supernatants for ELISA analysis were collected from unstimulated (CL-01 N or SKW N) or CD40L + Interleukin-4 (IL-4) stimulated (CL-01 S or SKW S) cells after 4 days of incubation. For each figure, the Y-axis represents the antibody levels normalized to the naïve unstimulated cell control (CL-01 N or SKW N). CFC denotes the carbon fiber cloth control. Statistical significance was measured by two-way ANOVA analysis and Bonferroni post-test. Significant differences from the corresponding cell controls were denoted by * and ***, which represent significance at p < 0.05 and p < 0.001, respectively. Results are representative of three independent experiments (n = 3 for each treatment group). Error bars denote SE.