Figure 10.

Culturing HaCaT and B cells on scaffolds have modest effects on cytokine secretion. (A) HaCaTs were cultured for 5 days on long or short CNT scaffolds, and (B) naïve unstimulated CL-01 cells (CL-01 N), stimulated CL-01 cells (CL-01S), naïve unstimulated SKW cells (SKW N), or stimulated SKW cells (SKW S) were cultured for 4 days with long or short CNT scaffolds. CFC denotes the carbon fiber cloth control. Cytokine levels were measured with a Luminex 25-Plex Human ProcartaPlex™ Panel 1B (Invitrogen) assay. Only those cytokines that exhibited any significant changes are shown. The Y-axis represents the cytokine fold change compared to the naïve unstimulated cell control (N). Statistical significance was measured by two-way ANOVA analysis followed by Dunnett’s multiple comparisons test comparing each sample to the corresponding cell control. Significant difference from the cell control is denoted by *, **, ***, and ****, which represents significance at p < 0.05, p < 0.01, p < 0.001, and p < 0.0001, respectively. Results are representative of one experiment (n = 3 for each treatment group). Error bars denote SE.