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. 2023 Jun 2;11(6):e006388. doi: 10.1136/jitc-2022-006388

Figure 3.

Figure 3

Intrinsic Arg biosynthesis can support MYXV replication in Arg limited conditions. (A) Western blots analyzing expression of the urea cycle components ASS1, ASL, and OTC in B16F10 and A9F1 cells. (B) MTT assays of B16F10 and A9F1 cells cultured for 24 hours in control DMEM (control), Arg-deficient media supplemented with 400 µM citrulline (−Arg/+Cit), or Arg-deficient media (−Arg). (C) The indicated cells were incubated as previously shown and then infected with MyxGFP at an MOI=5. Twenty-four hours post infection, the amount of infectious virus present was quantified using viral titration assays. Statistical significance (B, C) was determined by analysis of variance with Tukey’s HSD post hoc test (n=3 per group, per cell line). ***P<0.001. Arg, arginine; ASL, argininosuccinate lyase; ASS1, argininosuccinate synthetase 1; DMEM, Dulbecco’s Modified Eagle Medium; FFU, focus-forming unit; HSD, honest significant difference; MyxGFP, myxoma virus-expressing green fluorescent protein; MYXV, myxoma virus; n.s., no significance; OTC, ornithine transcarbamylase.