Figure 4.

Loss of ASS1 reduces MYXV replication in vivo: ASS1^WT or ASS1^KO (KO#1 and KO#4) B16F10 tumors were established on C57Bl/6J mice and then treated with a single bolus of 1×10⁶ FFU of MyxGFP. Six days post infection, tumors were harvested and processed for frozen sectioning and quantification of viral titer. (A) Images of tumor sections that displayed the median amount of infection within each cohort. GFP signal indicates area of infection. (B) Quantification of infection area in infected tissues (shown in A) displayed as a percent of total tumor area, which is GFP+. One section was taken for imaging per tumor/mouse (n=6–9 per group). Hashed bars indicate the mean value for each group. (C) Quantification of infectious virus present within tumors (n=6–9 per group). Hashed bars indicate the mean value for each group. Statistical significance (B, C) was determined by analysis of variance with Games-Howell post hoc test. *P<0.05, ***P<0.001. FFU, focus-forming unit; GFP, green fluorescent protein; MyxGFP, myxoma virus-expressing green fluorescent protein; MYXV, myxoma virus; n.s., no significance; WT, wild type.