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In order (1) SHBG antibody-staining and (2) PTMA antibody-staining. (a,e) larva unexposed to TiO2-NPs; (b,f) larva exposed to 1 mg/L TiO2-NPs; (c,g) larva exposed to 2 mg/L TiO2-NPs; and (d,h) larva exposed to 4 mg/L TiO2-NPs. The histogram next to each photo represents the average fluorescence intensity (AU) of the corresponding biomarker. Scale bar 420 µm.
