Skip to main content
. 2023 Jun 7;43(23):4251–4261. doi: 10.1523/JNEUROSCI.1668-22.2023

Figure 4.

Figure 4.

Chemogenetic activation of GC GLP-1R+ neurons reduces dark-cycle chow intake. A, Example image of DREADD expression (YFP+) in the GC from one mouse. Scale bar, 50 µm. B, Shaded regions illustrating representative spread of AAV-DIO-hM3D(Gq)-mCitrine expression within the GC. Anterior–posterior (AP) coordinates are relative to bregma. Arrow indicates example mouse shown in A. C, Schematic of experimental timeline. D, Effect of DREADD ligand J60 (0.1 mg/kg, i.p.) on dark-cycle chow intake in Glp1r-Cre mice bilaterally expressing GC AAV-DIO-hM3D(Gq)-mCitrine, and in Glp1r-Cre mice that do not express DREADD receptors. Mean + SEM of chow consumed (corrected to kcal consumed divided by body weight); n = 7/group, 3 females, 4 males (blue circles, males; pink circles, female; *p < 0.05 vs vehicle).