Figure 1.

ID8Cosmc-KO as a model for ovarian cancer in C57BL/6J mice. Tn antigen expression on ID8Cosmc-KO was measured by flow cytometry using either (A) staining with monoclonal mouse IgM anti-Tn antibody 5F4 followed by goat anti-mouse IgM conjugated to Alexa Fluor 647 or (B) biotinylated anti-Tn-OTS8 WE scFv tetramer containing streptavidin-PE. (C) ID8 WT and ID8Cosmc-KO cell doubling times (19.2 hours and 21.8 hours, respectively) in vitro were measured over time by manual cell counting. Growth curves from two different experiments (total n=6) were combined and plotted using GraphPad Prism V.9.4.1 and cell doubling times were calculated using the exponential growth equation. (D) Timeline for ID8Cosmc-KO tumor growth in syngeneic C57BL/6J mice after intraperitoneal injection of 10⁷ tumor cells. (E) Survival curves of C57BL/6J mice inoculated intraperitoneally with 10⁷ ID8 WT or ID8Cosmc-KO. Median survival was 88 days and 73 days for ID8 WT (n=19) and ID8Cosmc-KO (n=35), respectively. For C57BL/6 Rag1-KO mice inoculated with 10⁷ ID8Cosmc-KO (n=10), the median survival was 28 days. Survival curves were plotted using GraphPad Prism V.9.4.1 and effects were calculated using the log-rank (Mantel-Cox) test. i.p., intraperitoneal; KO, knock-out; scFv, single-chain Fv; WT, wild-type.