Table 3.
qPCR constituents.
| Reaction Reagent | Sample Volume |
|---|---|
| 2× ChamQ SYBR qPCR Master Mix | 10 μL |
| Primer1 (10 μM) | 0.4 μL |
| Primer2 (10 μM) | 0.4 μL |
| 50× ROX Reference Dye 1 | 0.4 μL |
| Template DNA/cDNA | 2.0 μL |
| Double distilled H2O | 6.8 μL |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.