Figure 2.

Deletion and mutational analyses of the hTERT core promoter. Luciferase reporter plasmids with serial deletions of the core promoter or substitution mutations in the factor binding sites were prepared. Crossed-out boxes indicate the mutated sites for binding factors shown above the figure. The mutated sequences are shown in Figure 1. These reporter plasmids were transfected into C33A, ME180 and SiHa cells, and luciferase assays were performed. The luciferase activity of the p181 wild-type plasmid was normalized to 100, and the relative luciferase activity is shown.