Figure 3.
Gel shift analyses representing Myc/Max binding to the E-box in the core promoter. Twenty micrograms of nuclear extracts from C33A cells (A) or various amounts of recombinant proteins (B) were subjected to gel shift assays using the E-box region at –165 as probe (Materials and Methods) in the presence or absence of competitors or antibodies. homo, homologous competitors; homo MT, homologous competitors in which E-box sequences are mutated; Myc/Max, Myc/Max consensus oligonucleotides; Myc/Max MT, Myc/Max consensus oligonucleotides in which E-box sequences are mutated. S indicates the supershifted bands.