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. 2000 Feb 1;28(3):669–677. doi: 10.1093/nar/28.3.669

Figure 4.

Figure 4

Figure 4

Figure 4

Figure 4

Figure 4

Myc/Max, Sp1 and Mad/Max induction assays. Various amounts of Max expression vectors together with c-Myc expression vectors were co-transfected with wild-type (p181) or mutant reporter plasmid Myc MT1+2 (see Fig. 2) into SiHa cells (A) or normal human foreskin keratinocytes (NHK) (B). Sp1 expression vectors were transfected with p181 or mutant reporter plasmid Sp1MT1–5 into normal human skin fibroblasts (NHF) (C). Mad1 expression vectors together with Max expression vectors were co-transfected with p181 or MycMT1+2 into C33A cells (D). c-Myc and Max expression vectors were also co-transfected with Sp1-MT1-5 into NHK cells (E). As a control, blank vectors in which cDNA sequences for c-Myc, Max, Mad1 or Sp1 were deleted were used. The luciferase activity in control samples was normalized to 1.0. The ratios of Myc/Max and Mad/Max introduced are shown below the figure. Bars indicate SD.