Figure 6.

Expression of c-Myc, Max and Sp1 in fibroblast lineages in different stages of transformation. Normal fibroblasts (RB) and transformants obtained by introduction of the SV40 LT were examined for expression of c-Myc, Max and Sp1 as well as transcriptional activity and telomerase activity. Myc, Max and Sp1 expression was examined by western blot analysis. β-Actin protein was measured to ensure even loading. The transcriptional activity in each lineage was examined by luciferase assay using reporter plasmid p1375. The transcriptional activity in RBT clones was normalized to 100, and the relative transcriptional activity is shown. The relative transcriptional activity of p1375 is shown for each lineage. Telomerase activity was examined by the TRAP assay.