Fig. 2. Heroin drives coordinated transcriptional patterning across brain reward regions that shifts with abstinence after a history of intake.

(A) Proportion of up-regulated (yellow) and down-regulated (blue) genes across brain regions for each experimental condition [heroin 24 hours (H24), saline-heroin (SH), heroin-saline (HS), and heroin-heroin (HH)]. (B) Number of shared DEGs identified across paired brain regions as being up-regulated (left) or down-regulated (right). (C) Genes identified as being regulated across three or more brain regions, with many showing coordinated expression changes across all regions identified. (D) GO analysis reveals that genes expressed across multiple brain regions enrich for biological processes related to various ECM processes including collagen, keratan sulfate, and chondroitin, in addition to iron homeostasis and cytokine and chemokine activity.